🔑 Key Takeaways
- Standard Epithalon dose: 5–10mg per day for 10–20 day courses
- Most protocols run 10 days on, then off for 3–6 months
- Can be injected subcutaneously or administered as nasal spray
- Results are cumulative — most people run 1–2 courses per year for longevity
- 10mg vial from Ascension = one full standard course at 1mg/day for 10 days
- Free calculator: Reconstitution Calculator
Epithalon (also spelled Epitalon) is a tetrapeptide studied for its effects on telomerase activation, sleep regulation, and longevity. Unlike most peptides taken daily in ongoing cycles, Epithalon is typically used in short intensive courses 1–2 times per year. Here's how to dose it properly.
Standard Epithalon Dosage Protocols
Protocol 1 — Standard Longevity Course (Most Common)
- Dose: 5–10mg total daily
- Split: 2.5–5mg twice daily (morning + evening) OR single daily injection
- Duration: 10 days on, then stop
- Frequency: 1–2 courses per year (e.g., spring and fall)
- Best for: general longevity, telomere support, anti-aging
Protocol 2 — Conservative Low-Dose Course
- Dose: 1–2mg per day
- Duration: 10–20 days
- Frequency: 1–2x per year
- Best for: first-timers, conservative approach
Protocol 3 — Sleep & Pineal Regulation
- Dose: 5mg before bed
- Duration: 10 days
- Frequency: 1x per year or as needed for sleep issues
- Best for: sleep quality, circadian rhythm regulation
Reconstitution reference — 10mg vial:
For a full 10-day standard protocol at 5mg/day, you need 5 vials. At 1mg/day for 10 days, one vial covers the course.
Nasal Spray Dosage
Epithalon is also used as a nasal spray:
- Typical concentration: 1–2mg per mL
- Dose: 2–4 sprays per nostril, 1–2x daily
- Less precise than injection but more convenient
- Lower bioavailability than subcutaneous injection (estimated 60–80%)
- Best for: people avoiding injections
Injectable Epithalon — Subcutaneous Protocol
- Injection site: abdomen, outer thigh, or upper arm
- Needle: 29–31G insulin syringe, 0.5 inch
- Inject slowly (5–10 seconds)
- Rotate sites between doses
- Most people inject once daily in the evening — Epithalon has pineal/sleep effects, and evening timing makes sense
Why Epithalon Is Dosed in Courses (Not Continuously)
Epithalon works differently from most peptides. Its primary studied mechanism is telomerase activation — stimulating the enzyme that rebuilds telomere length. This is not something that needs daily ongoing stimulation. Research protocols used short courses because:
- Telomere effects are cumulative and persist after the course
- Pineal gland regulation benefits carry over
- Receptor desensitization is avoided
- This is simply how it was studied in clinical research
Most longevity-focused users run 2 courses per year: once in spring, once in autumn.
What to Expect and When
- Days 1–3: possible mild changes in sleep quality, vivid dreams
- Days 4–7: some users report improved sleep depth and waking more refreshed
- Week 2–4 post-course: energy and general wellbeing may gradually improve
- Months later: the longevity and telomere effects are not something you "feel" — they're background biology
- Hair and skin: some users report improvements in hair growth and skin quality over months
Stacking Epithalon
Commonly stacked with:
- MOTS-C — mitochondrial peptide for energy and metabolic health. Complementary longevity stack.
- BPC-157 — for tissue repair alongside longevity support
- GHK-Cu — skin, hair, and anti-aging from a different angle (collagen, copper peptide)
- Not typically stacked with GHRPs or GLP-1s — different purpose
The information in this article is for educational purposes only and does not constitute medical advice. Always consult a healthcare professional before starting any new supplement or compound. Results vary by individual.