FOXO4-DRI Dosage Chart & Schedule Guide.
FOXO4-DRI (Proxofim) is a D-retro-inverso senolytic peptide that selectively clears senescent "zombie" cells by disrupting the FOXO4-p53 survival interaction. Based on the landmark Baar et al. (Cell, 2017) study, it is used in pulse cycles to restore tissue homeostasis.
Half-Life
~2–6 hours
Injection
Subcutaneous
Protocol
Pulse / Daily
Storage
−20 °C
Pulse Protocol
The research-backed approach: 3 subcutaneous injections on alternating days (Mon / Wed / Fri), repeated quarterly. Mirrors the mouse study design adapted for subcutaneous administration.
Use the calculator below to find your exact draw volume based on your vial and reconstitution.
1.5 mg
per injection · SC
Schedule
Every other day × 3
Pulse Total
4.5 mg
Cadence
Quarterly
1.5 mg/inj
3 mg
per injection · SC
Schedule
Every other day × 3
Pulse Total
9 mg
Cadence
Quarterly
3 mg/inj
Pulse Window
Inject on Mon / Wed / Fri (every other day × 3 doses over 5 days), then stop. Repeat the full pulse quarterly — 3 to 4 times per year.
| Protocol | Mon | Tue | Wed | Thu | Fri | Sat | Sun |
|---|---|---|---|---|---|---|---|
| Low Pulse | |||||||
| Standard Pulse |
Daily Titration Protocol
An alternative extended protocol used by some researchers: daily subcutaneous injections over 8–16 weeks with gradual dose escalation. Less studied than the pulse approach but allows for a slower, more controlled introduction.
0.25 mg
per injection · SC
Frequency
1× / day
Purpose
Adaptation phase
0.25 mg/day
0.375 mg
per injection · SC
Frequency
1× / day
Purpose
Dose escalation
0.375 mg/day
0.5 mg
per injection · SC
Frequency
1× / day
Purpose
Maintenance
0.5 mg/day
FOXO4-DRI Reconstitution Calculator
Enter your vial size, bacteriostatic water added, and target dose. A 10 mg vial with 3 mL water gives a concentration of 3.33 mg/mL.
Mid-size, most popular for peptides
Concentration
3.33 mg/mL
Draw volume
0.075 mL
= 0.25 mg ÷ 3.33 mg/mL
Units to draw (0.5 mL syringe)
8 units
0.5 mL Syringe
8 IUTips & Notes
Pulse vs Daily — Which to Use
The pulse protocol (3 injections quarterly) is directly derived from the 2017 mouse research and is the most commonly referenced approach. The daily titration protocol is community-developed and offers a gentler, more controlled alternative with less data behind it.
Injection Sites
Inject subcutaneously into the abdomen, front of thigh, or upper arm. Rotate sites within each pulse cycle. Do not inject into the same site on consecutive injection days — allow at least 2 inches of separation.
Transient Inflammatory Response
As senescent cells undergo apoptosis, they release inflammatory mediators (SASP). A brief period of increased inflammation, fatigue, or mild flu-like symptoms in the days after a pulse is not uncommon and typically resolves within a week.
Storage
Store lyophilized (powder) vials at −20 °C until use. After reconstitution with bacteriostatic water, refrigerate at 2–8 °C and use within 14–28 days. Avoid repeated freeze-thaw cycles and keep away from light at all times.
Disclaimer: This content is for informational purposes only. FOXO4-DRI is an investigational compound with no completed human clinical trials. All human dosing is extrapolated from animal research. Always consult a qualified healthcare professional before starting any new compound. PeptideDeck is not responsible for individual use.