Wolverine Stack Dosage Chart.
The Wolverine Stack is BPC-157 and TB-500 run together for injury recovery, sold either as two separate vials or as one pre-blended vial at a 1:1 ratio. Because it is a blend, every dose has two numbers: what you draw, and what each peptide inside it actually amounts to. This chart gives both.
Blend vial: 1,000 mcg once daily (500 mcg of BPC-157 plus 500 mcg of TB-500), which is 10 units on a U-100 syringe from a 20 mg vial in 2 mL of bacteriostatic water. Separate vials: 250 mcg of BPC-157 daily and 2.5 mg of TB-500 twice weekly. Either way, a 4–6 week block. These are conventions, not trial findings.
This chart is the condensed version. For the full write-up (mechanism, stacking logic, vendor testing and the rest), read the full Wolverine Stack dosing guide.
Dosage Protocols
Units assume a 20 mg blend vial (10 mg of each peptide) in 2 mL bacteriostatic water = 10,000 mcg/mL, so 1 unit on a U-100 syringe is 100 mcg of blend: 50 mcg of each. Different vial or ratio? Use the calculator. The big number is the total blend you draw; the split line is what each peptide inside it comes to.
Sized so the BPC-157 side lands exactly on its own conventional 250 mcg daily dose.
Why this dose+
The trade-off is on the other side of the vial: 1,750 mcg of TB-500 a week is well under the 4–5 mg weekly figure TB-500 protocols normally use. Two weeks here tells you whether you tolerate the injections before you commit a vial.
The dose most blend protocols converge on, and the closest a 1:1 vial gets to satisfying both peptides.
Why this dose+
500 mcg of BPC-157 a day sits inside the range people use for an active injury, and 3.5 mg of TB-500 a week lands just under the usual 4–5 mg loading figure. It also draws at a clean 10 units, which keeps measurement error small.
A front-loading block for the first few weeks after an acute strain, and the ceiling we would use.
Why this dose+
This is where the TB-500 side reaches the top of its conventional loading range at 5.25 mg a week, so it is a ceiling rather than a step on the way to something higher. It also empties a 20 mg vial in about 13 days.
- The milligrams on the label are the combined powder weight of two peptides.
- On a 1:1 vial, 1,000 mcg drawn is 500 mcg of BPC-157 plus 500 mcg of TB-500.
- "BPC-157 10 mg / TB-500 10 mg" is 20 mg of powder; "Wolverine Stack 10 mg" is usually 5 mg of each.
- Write down the total powder weight and the mg of each peptide before you add water.
- A fixed 1:1 ratio moves both peptides together: raise the blend and BPC-157 rises by as much.
- The acute tier stops at 1,500 mcg, where the TB-500 side tops its conventional weekly range.
- No human dose-response curve for healing exists for either peptide, by any route.
- To move one peptide independently, run separate vials rather than a bigger draw.
Weekly Schedule
A blend vial is injected daily, dosed to the BPC-157 side, with the TB-500 spread across seven small shots instead of two large ones. The last two rows keep each peptide on its own cadence instead.
Read the last two rows together. Separate vials cost nine injections a week rather than seven, and two vials to mix instead of one, but each peptide runs on the cadence its own protocol specifies and you can stop one without stopping the other. Pick one row and keep it identical week to week. Missed a day? Skip it and carry on.
Wolverine Stack Reconstitution Calculator
Enter your vial size, how much water you added, and the dose you want. We'll tell you exactly how much to draw. Enter the total powder weight of the blend: for a 10 mg / 10 mg vial that is 20, not 10. Every dose it returns is a total blend dose, so halve it for the per-peptide amount on a 1:1 vial.
Double-click a preset to edit it
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Ultra-fine, best for small doses
Concentration
10.00 mg/mL
1 unit = 100 mcg
Draw volume
0.100 mL
= 1000 mcg ÷ 10000 mcg/mL
Units to draw (0.3 mL syringe)
10.0 units
0.3 mL Syringe
10.0 IUFrom Vial to Injection in 6 Steps
Find out whether the number on the vial is the combined powder weight or the weight of each peptide. A vial sold as "BPC-157 10 mg / TB-500 10 mg" holds 20 mg of powder. A vial sold as "Wolverine 10 mg" usually holds 5 mg of each. Every calculation downstream depends on which one you have.
Alcohol swab the blend vial and the bacteriostatic water vial, and let both air-dry before you pierce them.
Run the bacteriostatic water down the inside wall of the vial rather than squirting it onto the powder cake. Two peptides means a larger cake than a single-peptide vial, so give it a moment.
Roll the vial gently until the solution is completely clear. Shaking damages peptides, and there is no reason to rush a step that takes thirty seconds.
Use a fresh U-100 insulin syringe and the calculator. On a 20 mg vial in 2 mL, every unit is 100 mcg of blend, 50 mcg of each peptide. So 500 mcg is 5 units, 1,000 mcg is 10 units and 1,500 mcg is 15 units.
People inject subcutaneously, often into the abdomen or near the injured area, rotating sites and staying clear of the navel, scar tissue and bruises. Keep the mixed vial at 2–8 °C, never freeze it, and treat roughly 28 days as the outside limit; that figure comes from the benzyl alcohol preservative in bacteriostatic water, not from any stability study of either peptide. Needles go in a sharps container.
- A 20 mg blend vial is 20 doses at the standard 1,000 mcg: about three weeks, inside the 28-day preservative window.
- At the conservative tier the same vial lasts 40 days, longer than a mixed solution should be kept.
- A 5 mg BPC-157 vial gives 20 daily doses at 250 mcg.
- A 10 mg TB-500 vial gives four doses at 2.5 mg: two weeks of the twice-weekly leg.
What to Expect
Each entry below is either something users consistently report or an explicit gap in the record.
The first week is about tolerance, not results. Neither peptide has published human pharmacokinetics for subcutaneous dosing, so nobody can tell you when a dose peaks or how long it lasts.
This is where self-reported stiffness and soreness usually shift, and where the acute front-loading block would normally end. The one published human report of BPC-157 in a musculoskeletal setting is a 16-patient case series of intra-articular knee injections with no placebo arm.
Four to six weeks is the conventional length of a Wolverine run, and the point to compare against something concrete you wrote down at the start: range of motion, load tolerated, pain at a named movement. If nothing has moved by week six, another six weeks of the same dose is unlikely to change that.
There is no human data on running either peptide for months, and none at all on running them together. The longest human exposure in this family is the thymosin β4 safety study, which dosed healthy volunteers intravenously for 14 days. Extended and repeated cycles are entirely convention.
Injuries heal on their own. No controlled human trial separates the peptide from the same four to six weeks of calendar. Write down one specific, repeatable measure before your first injection (degrees of range of motion, the load at which a movement hurts) and re-measure the same way at week three and week six.
Feeling Something? Check Here
- Redness, itching, a small bump or a mild sting at the injection site.
- A brief head-rush or light-headedness in the minutes after injecting.
- Mild nausea in the first few days.
- A little fatigue in the first week of a cycle.
- Fatigue that keeps building past the first week.
- Injection-site lumps that do not resolve.
- Palpitations or a racing heart.
- Persistent headaches.
- Light-headedness severe enough to affect standing or driving.
- Any new lump, mole change or unexplained symptom: both peptides act on angiogenesis and cell migration in animal models.
- Swelling of the face, lips or throat.
- Hives or a spreading rash.
- Difficulty breathing.
- Chest pain.
- Spreading redness, heat, pus or fever around an injection site, which is an infection rather than a peptide effect.
- Active malignancy, or an undiagnosed lump or lesion under investigation: both peptides act on angiogenesis and cell migration in animal models.
- Pregnancy or breastfeeding.
- Known hypersensitivity to either peptide, or to the benzyl alcohol in bacteriostatic water.
- Drug-tested sport: BPC-157 is prohibited at all times under WADA category S0, unapproved substances.
- Drug-tested sport: thymosin β4 and its TB-500 fragment are covered as a prohibited growth factor.
No trial-derived side-effect profile exists for either peptide. The absence of documented harms measures how little has been studied, not safety.
FAQ
Does the mg on a Wolverine vial mean each peptide or both together?+
It depends entirely on the vendor, which is exactly why this is the most expensive mistake on this page. A vial listed as "BPC-157 10 mg / TB-500 10 mg" contains 20 mg of powder, 10 mg of each. A vial listed as "Wolverine Stack 10 mg" almost always means 10 mg total, so 5 mg of each. Same printed number, half the peptide. Before you add water, work out the total powder weight and the amount of each peptide inside it, because the calculator, the concentration and every unit count depend on the total. If the listing does not say, ask the vendor and do not guess.
How many units of a U-100 syringe is 1,000 mcg of the blend?+
Ten units, on a 20 mg blend vial mixed with 2 mL of bacteriostatic water. That gives 10,000 mcg/mL, and one unit on a U-100 syringe is 0.01 mL, so one unit is 100 mcg of blend. On a 1:1 vial that unit is 50 mcg of BPC-157 and 50 mcg of TB-500. Change the vial size or the water volume and every number changes: a 10 mg vial in the same 2 mL makes each unit 50 mcg of blend, so 1,000 mcg becomes 20 units, not 10. Always recalculate from the vial in front of you.
Should I buy a pre-mixed blend or two separate vials?+
Separate vials, if you want each peptide dosed the way its own protocol says. BPC-157 convention is a small dose every day; TB-500 convention is a large dose twice a week. A fixed 1:1 blend cannot do both; whatever cadence you pick, one of the two peptides is off its usual schedule. Separate vials let you run BPC-157 at 250 mcg daily and TB-500 at 2.5 mg twice weekly, exactly as each chart specifies, and they let you stop one without stopping the other. The blend wins on convenience and on having one vial to mix instead of two. Neither arrangement has been compared to the other in a human study.
Why does the blend get injected daily when TB-500 is normally dosed twice a week?+
Because the blend is dosed to the BPC-157 side. BPC-157 is conventionally taken daily, so a 1:1 blend follows that cadence and the TB-500 comes along with it, spread across seven smaller injections instead of two large ones. At the standard tier that works out to 3.5 mg of TB-500 a week, which is close to the usual weekly total even though the pattern is different. Whether spreading it out matters is unknown; no study has compared dosing frequencies for TB-500 in humans, at any dose, by any route.
What happens if I use a TB-500 chart to dose a blend vial?+
You overdose the BPC-157 side badly. A TB-500 chart says 2.5 mg twice weekly. Draw 2.5 mg out of a 1:1 blend and you have injected 1.25 mg of TB-500 (half of what you intended) plus 1.25 mg of BPC-157, which is five times the conventional 250 mcg dose. Try to fix the TB-500 shortfall by drawing 5 mg of blend and you are now injecting 2.5 mg of BPC-157 in one shot, ten times the usual dose. This is the single commonest error people make with blends, and it comes from reading a single-peptide number off a single-peptide chart and applying it to a vial holding two.
Can I mix BPC-157 and TB-500 in the same syringe?+
People do it routinely, and there is no published compatibility or stability study for the combination: not for the pre-blended vials, and not for drawing two reconstituted peptides into one barrel. If you are running separate vials, the cleaner approach is two injections, because it keeps the doses independent and means a problem with one vial does not contaminate the other. If you do combine them, draw them one after the other into the same syringe and inject immediately rather than letting the mixture stand.
How long should a Wolverine cycle run?+
Four to six weeks at the standard dose is the block almost every protocol uses, sometimes with a two to three week front-load at the higher dose first. That number is convention, not a studied duration. It happens to line up with two practical constraints: a 20 mg vial at 1,000 mcg a day lasts 20 days, and a reconstituted vial should be finished inside about 28 days because of the preservative in bacteriostatic water. So a typical run is one to two vials. Set an end date before you start rather than deciding week by week.
Is the Wolverine Stack tested in humans?+
The combination has never been tested in humans. Neither has either component, for injury healing, by the subcutaneous route people actually use. Filtering PubMed for BPC-157 clinical trials returns zero published controlled trials; the human record is a Phase 1 study of oral tablets that posted no results, a 16-patient uncontrolled case series of intra-articular knee injections, and a Phase 2 trial for hamstring strain that is still recruiting. TB-500 itself (the Ac-LKKTETQ fragment) has no human trial at all; the human safety and pharmacokinetic data everyone quotes is for full-length recombinant thymosin β4 given intravenously, which is a different molecule by a different route.
Is it banned in competitive sport?+
Yes, both halves, and they are prohibited under different sections. BPC-157 falls under WADA category S0, unapproved substances, because it has no approval for human therapeutic use from any regulatory authority anywhere: prohibited at all times, in and out of competition. Thymosin β4 and its TB-500 fragment are covered as a prohibited growth factor. There is no therapeutic-use exemption available for a compound with no approved indication, and TB-500 metabolites have been characterised specifically for anti-doping detection in plasma and urine. If you are tested under the WADA code or any body that adopts it, this stack is disqualifying at any dose.
Sources
None of the doses on this page come from a trial. The record is registry entries and a case series for BPC-157, human data for full-length thymosin β4 rather than the TB-500 fragment sold under that name, and the chemistry establishing what TB-500 is. There is no study of the combination.
- ·PubMed search for "BPC 157" filtered to the Clinical Trial publication type returns 0 results, confirming there is no published controlled clinical trial of BPC-157.
- ·Lee E, Padgett B. Intra-articular injection of BPC 157 for knee pain. 2021. PMID 34324435. Uncontrolled case series, 16 evaluable patients, intra-articular route; no placebo arm.
- ·ClinicalTrials.gov record NCT02637284: Phase 1 of oral BPC-157 tablets (1 mg tablets, up to 9 mg/day), started 2015, completed, no results posted.
- ·ClinicalTrials.gov record NCT07437547: Phase 2 of subcutaneous BPC-157 for hamstring strain, recruiting from 2026; the registry record does not disclose the dose.
- ·Synthesis and characterization of the N-terminal acetylated 17-23 fragment of thymosin beta 4 identified in TB-500. Drug Test Anal. 2012. PMID 22962027. Chemically identifies TB-500 as Ac-LKKTETQ, a fragment rather than the full-length protein, and describes plasma and urine detection.
- ·Ruff D, Crockford D, Girardi G, Zhang Y. A randomized, placebo-controlled, single and multiple dose study of intravenous thymosin beta 4 in healthy volunteers. Ann N Y Acad Sci. 2010. PMID 20536472. 42–1,260 mg IV, single dose then the same dose daily for 14 days, no dose-limiting toxicity. Full-length Tβ4, not TB-500.
- ·First-in-human, randomized, double-blind, single- and multiple-dose Phase I study of recombinant human thymosin β4. J Cell Mol Med. 2021. PMC8419156. 0.05–25 µg/kg IV; terminal half-life 0.5–2.08 h, dose-dependent.
- ·Simultaneous quantification of TB-500 and its metabolites in vitro and in rats. J Chromatogr B. 2024. PMID 38382158. States that the biological effects of TB-500 have not been documented; Ac-LKKTE was the only metabolite with wound-healing activity in fibroblasts.
- ·Therapeutic Peptides in Orthopaedics: Applications, Challenges, and Future Directions. JAAOS Glob Res Rev. 2026. PMC12753158. TB-500 is usually injected subcutaneously or intramuscularly; the evidence base is preclinical and veterinary, with human orthopaedic data lacking.
- ·Mendias CL, Awan TM. Safety and Efficacy of Approved and Unapproved Peptide Therapies for Musculoskeletal Injuries and Athletic Performance. Sports Med. 2026. PMID 41966639. Reports favourable tissue-repair outcomes for this peptide class in animal models, and frames these compounds in anti-doping terms.
- ·USADA: BPC-157 is prohibited at all times under WADA category S0, Unapproved Substances, and is "not approved for human clinical use by any global regulatory authority".
- ·Discovery of thymosin β4 as a human exerkine and growth factor. Am J Physiol Cell Physiol. 2021. PMID 34495765. Notes the WADA classification of thymosin β4 as a prohibited growth factor.
- ·FDA label for Bacteriostatic Water for Injection USP (Hospira), via DailyMed: 0.9% (9 mg/mL) benzyl alcohol preservative, the source of the 28-day convention for a mixed vial.
The longer treatment of all of this (how the two peptides are thought to complement each other, vendor testing, and what to look for in a blend listing) lives in the full Wolverine Stack dosing guide.
Disclaimer: This content is for informational and research purposes only and is not medical advice. Neither BPC-157 nor TB-500 is an approved medicine, and the combination has never been studied in humans. Every dose here is convention rather than a trial-derived figure. Both are prohibited in competitive sport under the WADA code. Always consult a qualified healthcare professional before starting any peptide protocol. PeptideDeck is not responsible for individual use.