Peptide Reconstitution Calculator
Calculate exact peptide concentrations, volume per dose, and insulin syringe units when mixing lyophilized research peptides with bacteriostatic water.
- Wipe the vial top and BAC water vial with an alcohol swab
- Draw 2mL of bacteriostatic water into a syringe
- Inject slowly into the peptide vial — angle against the glass, don't spray on powder
- Gently swirl (never shake) until fully dissolved
- Label vial with date. Store in refrigerator (2–8°C). Use within 28–30 days.
- For each dose, draw to 20 IU on a U-100 insulin syringe
For research purposes only. Not medical advice. Always verify calculations independently.
How to Use This Reconstitution Calculator
When working with lyophilized (freeze-dried) research peptides, reconstitution is the process of adding bacteriostatic water (BAC water) to dissolve the powder into a liquid solution. Getting the math right is critical for accurate dosing.
Three inputs, four outputs
- Vial size (mg): The total amount of peptide powder in your vial — usually printed on the label (e.g., 5mg, 10mg, 30mg)
- BAC water volume (mL): How much bacteriostatic water you're adding to the vial
- Dose per injection (mg): The amount of peptide you want per dose
The calculator instantly shows concentration (mg/mL), the exact mL to draw per dose, how many doses your vial contains, and the insulin unit (IU) marker on a U-100 syringe.
Common Reconstitution Examples
| Peptide | Vial Size | BAC Water | Concentration |
|---|---|---|---|
| BPC-157 | 5mg | 2mL | 2.5mg/mL |
| TB-500 | 10mg | 2mL | 5mg/mL |
| Retatrutide | 10mg | 2mL | 5mg/mL |
| Semaglutide | 5mg | 2mL | 2.5mg/mL |
| Ipamorelin | 5mg | 2mL | 2.5mg/mL |
| CJC-1295 | 5mg | 2mL | 2.5mg/mL |
Storage After Reconstitution
Once reconstituted, store your peptide solution in the refrigerator (2–8°C / 36–46°F). Most research peptides remain stable for 28–30 days after reconstitution. Never freeze a reconstituted solution.