Complete Guide to Peptide Reconstitution and Storage

Proper reconstitution is one of the most fundamental skills for anyone working with research peptides. Lyophilized (freeze-dried) peptides must be carefully mixed with an appropriate solvent before use, and mistakes in this process can render expensive compounds inactive or unsafe.

This guide provides step-by-step instructions for reconstituting peptides correctly, calculating dosages from reconstituted solutions, and storing peptides to maximize their shelf life and potency.

Essential Supplies

Before reconstituting peptides, gather all necessary materials:

Bacteriostatic Water vs. Sterile Water

This distinction is critical:

Other Reconstitution Solvents

Some peptides require specific solvents. Common alternatives include:

• Acetic Acid (0.6% solution): Required for certain peptides that don't dissolve well in water (e.g., some GH fragments)
• Sodium Chloride 0.9% (Saline): Used for some pharmaceutical peptides
• DMSO: Rarely needed; only for specific research applications

For most common peptides (BPC-157, TB-500, Ipamorelin, CJC-1295, etc.), bacteriostatic water is the appropriate choice.

Reconstitution Process

Follow these steps carefully for safe and effective reconstitution:

Dosage Calculations

Understanding how to calculate your concentration and doses is essential. Here's the math:

Basic Formula

Common Reconstitution Examples

Insulin Syringe Units

Insulin syringes are marked in "units" where:

• 100 units = 1mL
• 10 units = 0.1mL
• 1 unit = 0.01mL

So if your concentration is 2,500mcg/mL and you want 250mcg, you need 0.1mL = 10 units on the syringe.

Calculation Shortcut

Proper Storage

Correct storage is crucial for maintaining peptide potency:

Before Reconstitution (Lyophilized Powder)

After Reconstitution

*Note: Freezing reconstituted peptides is debated. Some peptides tolerate freeze-thaw cycles, others may degrade. When possible, reconstitute only what you'll use within 4 weeks.

Signs of Degradation

Discard peptides if you notice:

• Cloudiness: Clear solutions turning cloudy or hazy
• Particulates: Visible floaters or sediment
• Color change: Solution becoming yellow, brown, or any unexpected color
• Unusual odor: Any smell (properly reconstituted peptides are odorless)
• Clumping: Powder that won't dissolve even with extended time

Reconstitution Mistakes

Avoid these common errors that can damage peptides or compromise safety:

❌ Mistake 1: Shaking the Vial

Vigorous shaking creates foam and can denature the peptide through mechanical stress. Always swirl gently or simply let the solution sit.

❌ Mistake 2: Spraying Water Directly on Powder

Forceful water contact can damage the peptide. Always drip water down the vial wall slowly.

❌ Mistake 3: Using Sterile Water Instead of Bacteriostatic Water

Without preservative, bacteria will grow in your multi-use vial, creating contamination risk.

❌ Mistake 4: Room Temperature Storage

Reconstituted peptides degrade rapidly at room temperature. Always refrigerate immediately after mixing.

❌ Mistake 5: Reusing Needles

Each puncture of the rubber stopper should use a new sterile needle to prevent contamination.

❌ Mistake 6: Not Labeling Vials

Without labels, you can't track concentration or reconstitution date. Always label immediately.

❌ Mistake 7: Reconstituting Too Far in Advance

Only reconstitute what you'll use within 3-4 weeks. Unreconstituted peptides have much longer shelf lives.

Frequently Asked Questions

Summary Checklist

Proper reconstitution technique ensures your peptides remain potent and safe throughout their use. Take the time to do it right—your research results depend on it.